国产美女高潮无套久久,无码毛片视频一区二区本码,芒果视频在线观看,亚洲无码午夜免费精品,我和我的老熟女视频,亚洲免费美女动漫精品二区,爆乳放荡的女教师在线观看,国产真人一级a爱做片无码,久久9991电影黄片,日本欧美色三级18禁黄网站,亚洲欧美婬色多多,加勒比熟妇中文高清

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > CRE BAG 2
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
CRE BAG 2
CRE BAG 2
規(guī)格:
貨期:
編號:B162306
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 CRE BAG 2
商品貨號 B162306
Organism Mus musculus, mouse
Tissue embryo
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Leukemia
Strain NIH/Swiss
Applications
produces protein beta-galactosidase beta-D-galactosidase
Derivation
The CRE BAG 2 cell line has never produced a replication competent helper virus.
The cells will package vectors derived from murine leukemia viruses.
Genes Expressed
a beta galactosidase transducing vector (BAG),beta-galactosidase(beta-D-galactosidase) [lacZ],beta-galactosidase
Cellular Products
a beta galactosidase transducing vector (BAG)
Comments
The BAG construct has been used to transfect psiCRE cells in ATCC CRL-1858 and to transfect psi2 cells in ATCC CRL-9560.
The CRE BAG 2 cell line has never produced a replication competent helper virus.
This line produces a vector (BAG) that can infect mouse and rat cells and transduce beta-galactosidase activity.
The line is similar to the psi 2 BAG alpha line (see ATCC CRL-9560) but has never been known to produce a replication competent helper virus.
The cells will package vectors derived from murine leukemia viruses.
The vector has been used to infect a variety of neural cell types and to express beta-galactosidase and resistance to G418.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin - 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor CL Cepko
References

Price J, et al. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84: 156-160, 1987. PubMed: 3099292

Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature 328: 131-136, 1987. PubMed: 3600789

Danos O, Mulligan RC. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc. Natl. Acad. Sci. USA 85: 6460-6464, 1988. PubMed: 3413107

Cepko CLVirus propagation and Xgal proceduresIn: Cepko CLNeuromethodsClifton, NJHumana Press1989.

Le Doux JM, et al. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection. J. Virol. 70: 6468-6473, 1996. PubMed: 8709284

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
西乡县| 长丰县| 临沂市| 米易县| 明光市| 宕昌县| 安义县| 凤翔县| 克东县| 丰城市| 宁阳县| 永州市| 商城县| 开化县| 灵石县| 凌源市| 乌拉特前旗| 孟州市| 利辛县| 永年县| 榕江县| 新民市| 克拉玛依市| 循化| 浪卡子县| 衡阳县| 如东县| 贵德县| 南安市| 咸阳市| 彰化县| 霍林郭勒市| 长宁县| 故城县| 罗定市| 若羌县| 乌审旗| 东阳市| 阿拉善右旗| 莱阳市| 新化县|