產(chǎn)品名稱(chēng) |
Human echovirus 9 |
商品貨號(hào) |
B162821 |
Classification |
Picornaviridae, Enterovirus, Enterovirus B |
Deposited As |
Echovirus 9 |
Agent |
Human echovirus 9 |
Strain |
Vispo |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1.0 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
Derived by adaptation of primary cell culture product ATCC® VR-1051™ to LLC-MK2 Derivative cells (ATCC® CCL-7.1™). ATCC® VR-1888™ and ATCC® VR-1051™ have not been compared for sequence or infectivity in primary cell culture and tissue culture.
The original source material has been reported to contain Simian virus 40. |
Effect on Host |
CPE, refractile rounding and sloughing |
Recommended Host |
LLC-MK2 Derivative cells (ATCC® CCL-7.1™) |
Growth Conditions |
Temperature: 36°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 36°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 3-4 days at 36°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer. |
Name of Depositor |
NIH/NIAID |