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Chlamydia muridarum Everett et al.
Chlamydia muridarum Everett et al.
規(guī)格:
貨期:
編號(hào):B163298
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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DNA
RNA

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甘油
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產(chǎn)品名稱 Chlamydia muridarum Everett et al.
商品貨號(hào) B163298
Classification Chlamydiaceae
Deposited As Chlamydia trachomatis (Busacca) Rake
Agent Chlamydia muridarum Everett et al.
Strain Mouse Pneumonitis strain Nigg II
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Storage Conditions -70°C or colder
Comments
Although this agent is not known to be pathogenic for man, it should be handled with care. Mice receiving 1:100 dilution i.n. of infected y.s. occasionally develop pneumonia and die.
The Nigg II strain was first deposited by F. B. Gordon as yolk-sac adapted material. It was subsequently redeposited as tissue culture adapted material by J. Schachter
This preparation has tested positive for Mycoplasma contamination
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Recommended Host
McCoy (ATCC® CRL-1696™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: 48 hours at 37°C in McCoy cells (ATCC® CRL-1696™). Add glassbeads and vortex preparation to disrupt cells.  Infect monolayer with disrupted material.  Centrifuge at 3000 x rpm (750 x g) for 1 hour.  Feed with fresh growth medium containing 1-2 µg/mL cycloheximide.  Incubate at 37°C for 48 hours.
Incubation: 48 hours
Name of Depositor FB Gordon, J Schachter
Source Normal mice, 1939
Year of Origin 1939
References

Pal S, et al. Immunization with an acellular vaccine consisting of the outer membrane complex of Chlamydia trachomatis induces protection against a genital challenge. Infect. Immun. 65: 3361-3369, 1997. PubMed: 9234798

Nigg C. An unidentified virus which produces pneumonia and systemic infection in mice. Science 95: 49, 1942.

Pal S, et al. Intranasal immunization induces long-term protection in mice against a Chlamydia trachomatis genital challenge. Infect. Immun. 64: 5341-5348, 1996. PubMed: 8945586

Kaushic C, et al. Chlamydia trachomatis infection in the female reproductive tract of the rat: influence of progesterone on infectivity and immune response. Infect. Immun. 66: 893-898, 1998. PubMed: 9488372

Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462

type strain

Cross References

Nucleotide (GenBank) : AJ012177 Chlamydia muridarum partial rnpB gene for RNase P RNA subunit.

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